Chelsea manager Emma Hayes rotated her squad with seven changes from their previous Women's Super League victory over West Ham. Ajax faced a setback with 16-year-old midfielder Lily Yohannes suspended due to accumulating three yellow cards in the competition's first leg.
In the match, Ajax captain Sherida Spitse had a near miss in the first half with a header hitting the roof of Zecira Musovic's net from a short corner. Erin Cuthbert, leading the home team, came close to scoring, but her shot narrowly missed the post.